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University of Veterinary Medicine

3 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Methods to Study Lipid Alterations in Neutrophils and the Subsequent Formation of Neutrophil Extracellular Traps

Graham Brogden^*1,2, Ariane Neumann^*1,3, Diab M. Husein¹, Friederike Reuner^1,4, Hassan Y. Naim¹, Maren von Köckritz-Blickwede^1,4

¹Department of Physiological Chemistry, University of Veterinary Medicine Hannover, ²Fish Disease Research Unit, University of Veterinary Medicine, ³Department of Clinical Sciences, Biomedical Center, Lund University, ⁴Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover

Lipids are known to play an important role in cellular functions. Here, we describe a method to determine the lipid composition of neutrophils, with emphasis on the cholesterol level, by using both HPTLC and HPLC to gain a better understanding of the underlying mechanisms of neutrophil extracellular trap formation.

Behavior

Systematic Assessment of Well-Being in Mice for Procedures Using General Anesthesia

Katharina Hohlbaum^1,2, Bettina Bert^2,3, Silke Dietze², Rupert Palme⁴, Heidrun Fink², Christa Thöne-Reineke¹

¹Institute of Animal Welfare, Animal Behavior and Laboratory Animal Science, Department of Veterinary Medicine, Freie Universität Berlin, ²Institute of Pharmacology and Toxicology, Department of Veterinary Medicine, Freie Universität Berlin, ³German Federal Institute for Risk Assessment (BfR), ⁴Unit of Physiology, Pathophysiology and Experimental Endocrinology, Department of Biomedical Sciences, University of Veterinary Medicine

We developed a protocol to assess well-being in mice during procedures using general anesthesia. A series of behavioral parameters indicating levels of well-being as well as glucocorticoid metabolites were analyzed. The protocol can serve as a general aid to estimate the degree of severity in a scientific, animal-centered manner.

JoVE Core

A Micro-agar Salt Bridge Electrode for Analyzing the Proton Turnover Rate of Recombinant Membrane Proteins

Jürgen Kreiter¹, Elena E. Pohl¹

¹Department of Physiology and Biophysics, University of Veterinary Medicine

In electrophysiological measurements, the presence of a diffusion potential disturbs the precise measurement of the reverse potential by altering the electrode potential. Using a micro-agar salt bridge, the impact of the diffusion potential is minimized, which allows a more precise measurement of substrate turnover numbers of reconstituted recombinant membrane proteins.