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The University of Texas Health Science Center at San Antonio

3 ARTICLES PUBLISHED IN JoVE

Medicine

Visualization of Streptococcus pneumoniae within Cardiac Microlesions and Subsequent Cardiac Remodeling

¹Department of Microbiology and Immunology, The University of Texas Health Science Center at San Antonio

Streptococcus pneumoniae forms discrete non-purulent microscopic lesions in the heart. Outlined is the protocol for a murine model of cardiac microlesion formation. Instruction is provided on microlesion visualization using microscopy, discrimination between early and late microlesions, and methods to detect cardiac remodeling in hearts of convalescent animals.

Medicine

Quality-Controlled Sputum Analysis by Flow Cytometry

Marcia Grayson^*1, Shao-Chiang Lai^*1, Lydia H. Bederka¹, Patricia Araujo¹, Jamila Sanchez¹, Xavier T. Reveles¹, Vivienne I. Rebel^1,2, Jennifer Rebeles¹

¹bioAffinity Technologies, ²Department of Cell Systems & Anatomy, The University of Texas Health Science Center at San Antonio

This protocol describes an efficient method for dissociating sputum into a single cell suspension and the subsequent characterization of cellular subsets on standard flow cytometric platforms.

Biochemistry

Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins

Courtney N. Johnson¹, David S. Libich¹

¹Department of Biochemistry and Structural Biology and Greehey Children’s Cancer Research Institute, The University of Texas Health Science Center at San Antonio

A protocol for the application of paramagnetic relaxation enhancement NMR spectroscopy to detect weak and transient inter- and intra-molecular interactions in intrinsically disordered proteins is presented.