Detection of SARS-CoV-2 Receptor-Binding Domain Antibody using a HiBiT-Based BioreporterReza Rezaei 1,2, Abera Surendran 1,2, Ragunath Singaravelu 1,2, Taylor R. Jamieson 1,2, Parisa Taklifi 3, Joanna Poutou 1,2, Taha Azad 1,2, Carolina S. Ilkow 1,2
1Ottawa Hospital Research Institute, 2Department of Biochemistry, Microbiology and Immunology, University of Ottawa, 3Department of Biotechnology, University of Tehran
The outlined protocol describes the procedure for producing the HiBiT-receptor-binding domain protein complex and its application for fast and sensitive detection of SARS-CoV-2 antibodies.
