Analysis of Single-cell Gene Transcription by RNA Fluorescent In Situ Hybridization (FISH)Elena Ronander 1,2, Dominique C. Bengtsson 1,2, Louise Joergensen 1,2, Anja T. R. Jensen 1,2, David E. Arnot 1,2,3
1Centre for Medical Parasitology, Department of International Health, Immunology & Microbiology, Faculty of Health Sciences, University of Copenhagen, 2Department of Infectious Diseases, Copenhagen University Hospital (Rigshospitalet), 3Institute of Infection and Immunology Research, School of Biology, University of Edinburgh
Fluorescent in situ hybridization (FISH) to identify mRNA transcripts in individual cells allows analysis of polygenic activity such as the simultaneous transcription of more than one member of the var multigene family in Plasmodium falciparum infected erythrocytes 1. The technique is adaptable and can be used on different types of genes, cells and organisms.
