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University Medical Center Freiburg

3 ARTICLES PUBLISHED IN JoVE

Biology

Studying Proteolysis of Cyclin B at the Single Cell Level in Whole Cell Populations

Dominik Schnerch¹, Marie Follo¹, Julia Felthaus¹, Monika Engelhardt¹, Ralph Wäsch¹

¹Department of Hematology, Oncology and Stem Cell Transplantation, University Medical Center Freiburg

Metaphase to anaphase transition is triggered through anaphase-promoting complex (APC/C)-dependent ubiquitination and subsequent destruction of cyclin B. Here, we established a system which, following pulse-chase labeling, allows monitoring cyclin B proteolysis in entire cell populations and facilitates the detection of interference by the mitotic checkpoint.

Medicine

Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease

Louise Benning¹, Samuel Robinson^1,2, Marie Follo³, Lukas Andreas Heger¹, Daniela Stallmann¹, Daniel Duerschmied¹, Christoph Bode¹, Ingo Ahrens^1,4, Marcus Hortmann¹

¹Department of Cardiology and Angiology I, Heart Center Freiburg University, Faculty of Medicine, University of Freiburg, ²Department of Medicine, Monash University, ³Department of Medicine I, Lighthouse Core Facility, Medical Center, University of Freiburg, ⁴Department of Cardiology, Augustinerinnen Hospital, Academic Teaching Hospital, University of Cologne

Circulating microRNAs have shown promise as biomarkers for cardiovascular diseases and acute myocardial infarctions. In this study, we describe a protocol for miRNA extraction, reverse transcription, and digital PCR for the absolute quantification of miRNAs in the serum of patients with cardiovascular disease.

Medicine

Erosion Identification in Metacarpophalangeal Joints in Rheumatoid Arthritis using High-Resolution Peripheral Quantitative Computed Tomography

Yousif Al-Khoury^1,2, Stephanie Finzel³, Camille Figueiredo⁴, Andrew J. Burghardt⁵, Kathryn S. Stok⁶, Lai-Shan Tam⁷, Isaac Cheng⁷, Justin J. Tse¹, Sarah L. Manske¹

¹Department of Radiology, McCaig Institute for Bone and Joint Health, University of Calgary, ²Department of Biomedical Engineering, University of Calgary, ³University Medical Center Freiburg, ⁴Department of Rheumatology, University of São Paulo, ⁵Department of Radiology and Biomedical Imaging, University of California, San Francisco, ⁶Department of Biomedical Engineering, University of Melbourne, ⁷Department of Medicine and Therapeutics, The Chinese University of Hong Kong

Bone erosions are an important pathological feature of rheumatoid arthritis. The purpose of this work is to introduce a training tool to provide users with guidance on identifying pathological cortical breaks on high resolution peripheral quantitative computed tomography images for erosion analysis.