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University of California San Diego School of Medicine

3 ARTICLES PUBLISHED IN JoVE

Medicine

Detection and Genogrouping of Noroviruses from Children's Stools By Taqman One-step RT-PCR

Sonia Apaza¹, Susan Espetia¹, Robert H. Gilman^1,2, Sonia Montenegro³, Susana Pineda³, Fanny Herhold¹, Romeo Pomari¹, Margaret Kosek², Nancy Vu¹, Mayuko Saito^1,2,4

¹Laboratorio de Investigación y Desarrollo (LID), Universidad Peruana Cayetano Heredia, ²Bloomberg School of Public Health, Johns Hopkins University, ³Laboratorio de Diagnostico Molecular, Facultad de Medicina, University of Concepcion,Chile, ⁴University of California San Diego School of Medicine

A One-Step RT-PCR assay for detection and genogroup identification of Norovirus isolates from children’s stools, that utilizes primers and TaqMan probes specific to the open reading frame 1 (ORF1)-ORF2 junction region, the most conserved region of the Norovirus genome is described. A non-commercial, cost-effective RNA extraction method is detailed.

Neuroscience

Characterizing the Composition of Molecular Motors on Moving Axonal Cargo Using "Cargo Mapping" Analysis

Sylvia Neumann¹, George E. Campbell^*1, Lukasz Szpankowski^*2,3, Lawrence S.B. Goldstein^2,4, Sandra E. Encalada¹

¹Department of Molecular and Experimental Medicine, Dorris Neuroscience Center, The Scripps Research Institute, ²Department of Cellular and Molecular Medicine, University of California San Diego, ³Department of Bioengineering, University of California San Diego, ⁴Department of Neurosciences, University of California San Diego School of Medicine

Intracellular transport of cargoes, such as vesicles or organelles, is carried out by molecular motor proteins that track on polarized microtubules. This protocol describes the correlation of the directionality of transport of individual cargo particles moving inside neurons, to the relative amount and type of associated motor proteins.

Immunology and Infection

A Murine Model of Group B Streptococcus Vaginal Colonization

Kathryn A. Patras¹, Kelly S. Doran^1,2

¹Department of Pediatrics, Division of Host-Microbe Systems & Therapeutics, University of California San Diego School of Medicine, ²Department of Biology and Center for Microbial Sciences, San Diego State University

The purpose of this protocol is to imitate human group B Streptococcus (GBS) vaginal colonization in a murine model. This method may be used to investigate host immune responses and bacterial factors contributing to GBS vaginal persistence, as well as to test therapeutic strategies.