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university of Johannesburg

3 ARTICLES PUBLISHED IN JoVE

Engineering

Angle-resolved Photoemission Spectroscopy At Ultra-low Temperatures

Sergey V. Borisenko¹, Volodymyr B. Zabolotnyy¹, Alexander A. Kordyuk^1,2, Danil V. Evtushinsky¹, Timur K. Kim^1,3, Emanuela Carleschi⁴, Bryan P. Doyle⁴, Rosalba Fittipaldi⁵, Mario Cuoco⁵, Antonio Vecchione⁵, Helmut Berger⁶

¹Institute for Solid State Research, IFW-Dresden, ²Institute of Metal Physics of National Academy of Sciences of Ukraine, ³Diamond Light Source LTD, ⁴Department of Physics, University of Johannesburg, ⁵CNR-SPIN, and Dipartimento di Fisica "E. R. Caianiello", Università di Salerno, ⁶Institute of Physics of Complex Matter, École Polytechnique Fédérale de Lausanne

The overall goal of this method is to determine the low-energy electronic structure of solids at ultra-low temperatures using Angle-Resolved Photoemission Spectroscopy with synchrotron radiation.

Chemistry

Achieving Efficient Fragment Screening at XChem Facility at Diamond Light Source

Alice Douangamath^*1,2, Ailsa Powell^*1,2, Daren Fearon^*1,2, Patrick M. Collins^1,2, Romain Talon^1,2,3, Tobias Krojer^3,4, Rachael Skyner^1,2, Jose Brandao-Neto^1,2, Louise Dunnett^1,2, Alexandre Dias^1,2, Anthony Aimon^1,2,3, Nicholas M. Pearce^1,3, Conor Wild^3,5, Tyler Gorrie-Stone¹, Frank von Delft^1,2,3,4,6

¹Diamond Light Source Ltd, Harwell Science and Innovation Campus, ²Research Complex at Harwell, Harwell Science and Innovation Campus, ³Structural Genomics Consortium, University of Oxford, ⁴Centre for Medicines Discovery, University of Oxford, ⁵Oxford Protein Informatics Group, Department of Statistics, Oxford University, ⁶Department of Biochemistry, University of Johannesburg

This paper describes the complete XChem process for crystal-based fragment screening, starting from applying for access and all subsequent steps to data dissemination.

Biology

Three-Dimensional Cell Culture of Adipose-Derived Stem Cells in a Hydrogel with Photobiomodulation Augmentation

Brendon Roets¹, Heidi Abrahamse¹, Anine Crous¹

¹Laser Research Centre, Faculty of Health Science, University of Johannesburg

Here, we present a protocol demonstrating the use of hydrogel as a three-dimensional (3D) cell culture framework for adipose-derived stem cell (ADSC) culture and introducing photobiomodulation (PBM) to enhance the proliferation of ADSCs within the 3D culture setting.