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University at Buffalo School of Medicine

4 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Bioluminescence Imaging of NADPH Oxidase Activity in Different Animal Models

Wei Han¹, Hui Li¹, Brahm H. Segal^2,3, Timothy S. Blackwell¹

¹Department of Medicine, Vanderbilt University School of Medicine, ²Departments of Medicine and Immunology, Roswell Park Cancer Institute, ³Department of Medicine, University at Buffalo School of Medicine

NADPH oxidase is the major source of reactive oxygen species (ROS) in phagocytes. Because of the ephemeral nature of ROS, it is difficult to measure and monitor ROS levels in living animals. A minimally invasive method for serial quantification of ROS in living mice is described.

Immunology and Infection

Oral Transmission of Listeria monocytogenes in Mice via Ingestion of Contaminated Food

Elsa N. Bou Ghanem¹, Tanya Myers-Morales¹, Grant S. Jones¹, Sarah E.F. D'Orazio¹

¹Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky

This paper describes a novel method for oral infection of mice using Listeria monocytogenes-contaminated food. The protocol can readily be adapted for use with other food borne bacterial pathogens.

Immunology and Infection

Assessing Anti-fungal Activity of Isolated Alveolar Macrophages by Confocal Microscopy

Melissa J. Grimm¹, Anthony C. D'Auria¹, Brahm H. Segal²

¹Department of Medicine, Roswell Park Cancer Institute, ²Department of Medicine, School of Medicine and Biomedical Sciences, University of Buffalo

A method to evaluate the ability of isolated mouse alveolar macrophages to control the growth of phagocytosed Aspergillus spores by confocal microscopy.

Immunology and Infection

A Mouse Model for the Transition of Streptococcus pneumoniae from Colonizer to Pathogen upon Viral Co-Infection Recapitulates Age-Exacerbated Illness

Alexsandra Lenhard^*1, Basma H. Joma^*2,3, Nalat Siwapornchai², Anders P. Hakansson⁴, John M. Leong^2,5, Elsa N. Bou Ghanem¹

¹Department of Microbiology and Immunology, University at Buffalo School of Medicine, ²Department of Molecular Biology and Microbiology, Tufts University School of Medicine, ³Graduate Program in Immunology, Tufts Graduate School of Biomedical Sciences, ⁴Department of Translational Medicine, Lund University, ⁵Stuart B. Levy Center for the Integrated Management of Antimicrobial Resistance, Tufts University

This paper describes a novel mouse model for the transition of pneumococcus from an asymptomatic colonizer to a disease-causing pathogen during viral infection. This model can be readily adapted to study polymicrobial and host-pathogen interactions during the different phases of disease progression and across various hosts.