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Jahangirnagar University

5 ARTICLES PUBLISHED IN JoVE

Neuroscience

Morphological Analysis of Drosophila Larval Peripheral Sensory Neuron Dendrites and Axons Using Genetic Mosaics

¹Disease Mechanism Research Core, RIKEN Brain Science Institute, ²Graduate School of Science and Engineering, Saitama University

The dendritic arborization sensory neurons of the Drosophila larval peripheral nervous system are useful models to elucidate both general and neuron class-specific mechanisms of neuron differentiation. We present a practical guide to generate and analyze dendritic arborization neuron genetic mosaics.

Neuroscience

Immunohistological Labeling of Microtubules in Sensory Neuron Dendrites, Tracheae, and Muscles in the Drosophila Larva Body Wall

Cagri Yalgin^1,2, M. Rezaul Karim^1,2, Adrian W. Moore¹

¹Disease Mechanism Research Core, RIKEN Brain Science Institute, ²Graduate School of Science and Engineering, Saitama University

To understand how complex cell shapes, such as neuronal dendrites, are achieved during development, it is important to be able to accurately assay microtubule organization. Here we describe a robust immunohistological labeling method to examine microtubule organization of dendritic arborization neuron sensory dendrites, trachea, muscle, and other Drosophila larva body wall tissues.

Neuroscience

Whole Mount Immunolabeling of Olfactory Receptor Neurons in the Drosophila Antenna

M. Rezaul Karim¹, Keita Endo², Adrian W Moore³, Hiroaki Taniguchi¹

¹Laboratory for Genetic Code, Graduate School of Life and Medical Sciences, Doshisha University, ²Laboratory for Circuit Mechanisms of Sensory Perception, RIKEN Brain Science Institute, ³Disease Mechanism Research Core, RIKEN Brain Science Institute

Herein we describe the process of whole mount immunostaining of Drosophila antennae, which enables us to better understand the molecular mechanisms involved in the diversification of olfactory receptor neurons (ORN)s.

Developmental Biology

Imaging of Cell Shape Alteration and Cell Movement in Drosophila Gastrulation Using DE-cadherin Reporter Transgenic Flies

M. Rezaul Karim¹, Tomohiro Haruta², Taro Matsumoto³, Hiroki Oda², Hiroaki Taniguchi^3,4,5

¹Biotechnology and Genetic Engineering Department, Jahangirnagar University, ²JT Biohistory Research Hall, ³Division of Cell Regeneration and Transplantation, School of Medicine, Nihon University, ⁴Graduate School of Life and Medical Sciences, Doshisha University, ⁵Institute of Genetics and Animal Breeding of the Polish Academy of Sciences

Herein we describe a procedure to capture live images of Drosophila gastrulation. This has enabled us to better understand the apical constriction involved in early development and further analyze mechanisms governing cellular movements during tissue structure modification.

Biochemistry

A Protein Preparation Method for the High-throughput Identification of Proteins Interacting with a Nuclear Cofactor Using LC-MS/MS Analysis

Megumi Tsuchiya^*1, M. Rezaul Karim^*2, Taro Matsumoto³, Hidesato Ogawa¹, Hiroaki Taniguchi^3,4,5

¹Graduate School of Frontier Biosciences, Osaka University, ²Department of Biotechnology and Genetic Engineering, Jahangirnagar University, ³Division of Cell Regeneration and Transplantation, School of Medicine, Nihon University, ⁴Institute of Genetics and Animal Breeding of the Polish Academy of Sciences, ⁵Graduate School of Life and Medical Sciences, Doshisha University

We have established a method for the purification of coregulatory interaction proteins using the LC-MS/MS system.