Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to EthanolPatricia J. Slininger 1, Maureen A. Shea-Andersh 1, Stephanie R. Thompson 1, Bruce S. Dien 1, Cletus P. Kurtzman 2, Leonardo Da Costa Sousa 3, Venkatesh Balan 3
1Bioenergy Research Unit, National Center for Agricultural Utilization Research, 2Mycotoxin Prevention and Applied Microbiology Research Unit, National Center for Agricultural Utilization Research, 3Chemical Engineering and Material Science, Great Lakes Bioenergy Center, Michigan State University
Adaptive evolution and isolation techniques are described and demonstrated to yield derivatives of Scheffersomyces stipitis strain NRRL Y-7124 that are able to rapidly consume hexose and pentose mixed sugars in enzyme saccharified undetoxified hydrolyzates and to accumulate over 40 g/L ethanol.
