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Erasmus MC, Rotterdam

2 ARTICLES PUBLISHED IN JoVE

Bioengineering

In Vivo Quantitative Assessment of Myocardial Structure, Function, Perfusion and Viability Using Cardiac Micro-computed Tomography

Elza van Deel^1,2, Yanto Ridwan¹, J. Nicole van Vliet¹, Sasha Belenkov³, Jeroen Essers^1,4,5

¹Department of Genetics, Erasmus MC, Rotterdam, ²Department of Experimental Cardiology, Erasmus MC, Rotterdam, ³PerkinElmer, Inc., ⁴Department of Vascular Surgery, Erasmus MC, Rotterdam, ⁵Department of Radiation Oncology, Erasmus MC, Rotterdam

This method outlines the use of Quantum Micro-Computed Tomography (MicroCT) to assess cardiac morphology, function, perfusion, metabolism and viability with iodinated contrast agent in mice with experimentally-induced myocardial ischemia. The technique can be applied for non-destructive high-throughput longitudinal in vivo imaging of various animal models of human heart disease.

Immunology and Infection

A Multimodal Imaging Approach Based on Micro-CT and Fluorescence Molecular Tomography for Longitudinal Assessment of Bleomycin-Induced Lung Fibrosis in Mice

Francesca Ruscitti¹, Francesca Ravanetti², Gaetano Donofrio², Yanto Ridwan³, Paula van Heijningen³, Jeroen Essers⁴, Gino Villetti¹, Antonio Cacchioli², Wim Vos⁵, Franco Fabio Stellari¹

¹Corporate Preclinical R&D, Chiesi Farmaceutici S.p.A., ²Department of Veterinary Science, University of Parma, ³Department of Molecular Genetics, Erasmus MC, ⁴Department of Molecular Genetics, Vascular Surgery, Radiation Oncology, Erasmus MC, ⁵Fluidda NV

We describe a non-invasive multimodal imaging approach based on Micro-CT and fluorescence molecular tomography for longitudinal assessment of the mouse lung fibrosis model induced by double intratracheal instillation of bleomycin.