A High-throughput Method for Measurement of Glomerular Filtration Rate in Conscious MiceTimo Rieg 1,2
1Division of Nephrology-Hypertension, Department of Medicine, University of California, San Diego , 2San Diego VA Healthcare System
Measurement of glomerular filtration rate (GFR) is the gold standard for kidney function assessment. Here we describe a high-throughput method which allows the determination of GFR in conscious mice by using a single bolus injection, determination of fluorescein isothiocyanate (FITC)-inulin in plasma and calculation of GFR by a two-phase exponential decay model.
