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New York State Department of Health

7 ARTICLES PUBLISHED IN JoVE

Biology

Using an EEG-Based Brain-Computer Interface for Virtual Cursor Movement with BCI2000

J. Adam Wilson¹, Gerwin Schalk², Léo M. Walton¹, Justin C. Williams¹

¹Department of Biomedical Engineering, University of Wisconsin-Madison, ²Wadsworth Center, New York State Dept. of Health

In this video, we demonstrate the steps required to run a brain-computer interface experiment, including setting up the EEG cap, calibrating the system, and training the user to move a cursor in two dimensions using imagined movements.

Neuroscience

Recording Human Electrocorticographic (ECoG) Signals for Neuroscientific Research and Real-time Functional Cortical Mapping

N. Jeremy Hill¹, Disha Gupta^1,2, Peter Brunner^1,2, Aysegul Gunduz^1,2, Matthew A. Adamo³, Anthony Ritaccio², Gerwin Schalk^1,2,4,5,6,7

¹Wadsworth Center, New York State Department of Health, ²Department of Neurology, Albany Medical College, ³Department of Neurosurgery, Albany Medical College, ⁴Department of Neurosurgery, Washington University, ⁵Department of Biomed. Eng., Rensselaer Polytechnic Institute, ⁶Department of Biomed. Sci., State University of New York at Albany, ⁷Department of Elec. and Comp. Eng., University of Texas at El Paso

We present a method for collecting electrocorticographic signals for research purposes from humans who are undergoing invasive epilepsy monitoring. We show how to use the BCI2000 software platform for data collection, signal processing and stimulus presentation. Specifically, we demonstrate SIGFRIED, a BCI2000-based tool for real-time functional brain mapping.

JoVE Methods Collections: Current Research Methods in Rabies Diagnosis, Prevention, Treatment, and Control

Charles E. Rupprecht¹, Wei-Cheng Hsu², Feng Ye³, Megan Golding⁴, Luca Zaeck⁵, Laurent Dacheux⁶, Noel Tordo⁷, Jodie A. Jarvis⁸, Erin Patrick⁹, Are Berentsen¹⁰

¹LYSSA LLC, ²Animal Health Research Institute, Council of Agriculture, Executive Yuan, ³Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary Medicine, Academy of Military Medical Sciences, ⁴Wildlife Zoonoses & Vector-Borne Diseases Research Group, Animal and Plant Health Agency, ⁵Institute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, ⁶Unit Lyssavirus Epidemiology and Neuropathology, National Reference Center for Rabies and WHO Collaborating Center for Reference and Research on Rabies, Institut Pasteur, ⁷Unit Antiviral Strategies, OIE Reference Laboratories for Rift Valley Fever Virus & Crimean Congo Hemorrhagic Fever Virus, Institut Pasteur, Institut Pasteur de Guinea, Conakry, ⁸Rabies Laboratory, Wadsworth Center, New York State Department of Health, ⁹Animal and Plant Health Inspection Service, Wildlife Services, Knoxville, TN, US Department of Agriculture, ¹⁰Animal and Plant Health Inspection Service, Wildlife Services, National Wildlife Research Center, US Department of Agriculture

JoVE Methods Collections: Current Research Methods in Rabies Diagnosis, Prevention, Treatment, and Control

Immunology and Infection

Isolating And Immunostaining Lymphocytes and Dendritic Cells from Murine Peyer's Patches

Magdia De Jesus¹, Sarita Ahlawat¹, Nicholas J. Mantis¹

¹Division of Infectious Diseases, New York State Department of Health

There is an increasing interest in understanding the immunological functions of specific subpopulations of cells in Peyer's patches (PPs), the primary inductive sites of gut-associated lymphoid tissues. Here we outline parallel protocols for preparing PP single cell preparations for flow cytometric analysis and PP cryosections for immunostaining.

Neuroscience

Rabies Necropsy Techniques in Large and Small Animals

Jodie A. Jarvis¹, Kathleen T. Brown¹, Kim A. Appler¹, D Patrick Fitzgerald¹, April D. Davis¹

¹Rabies Laboratory, Wadsworth Center, New York State Department of Health

The goal of this protocol is to demonstrate safe necropsy techniques in small and large animals to obtain satisfactory tissue samples for rabies testing.

Education

The Evoked Potential Operant Conditioning System (EPOCS): A Research Tool and an Emerging Therapy for Chronic Neuromuscular Disorders

N. Jeremy Hill^1,2, Disha Gupta^1,2, Amir Eftekhar¹, Jodi A. Brangaccio¹, James J. S. Norton^1,2, Michelle McLeod³, Tim Fake¹, Jonathan R. Wolpaw^1,2,4, Aiko K. Thompson³

¹National Center for Adaptive Neurotechnologies, Stratton VA Medical Center, ²Electrical & Computer Engineering Department, State University of New York at Albany, ³College of Health Professions, Medical University of South Carolina, ⁴Department of Biomedical Sciences, State University of New York at Albany

The Evoked Potential Operant Conditioning System aids scientific investigation of sensorimotor function and can administer targeted neurobehavioral training that can impact sensorimotor rehabilitation in neuromuscular disorders. This article describes its capabilities and illustrates its application in modifying a simple spinal reflex to achieve lasting improvement in motor function.

Immunology and Infection

Detection of Rabies IgG and IgM Antibodies Using the Rabies Indirect Fluorescent Antibody Test

Nicholas J. Jones¹, Jodie A. Jarvis¹, Kim A. Appler¹, April D. Davis¹

¹Rabies Laboratory, Wadsworth Center, New York State Department of Health

The aim of this manuscript is to examine the use of the rabies indirect fluorescent antibody test for the detection of rabies-specific IgG and IgM antibodies.