Consensus Brain-derived Protein, Extraction Protocol for the Study of Human and Murine Brain Proteome Using Both 2D-DIGE and Mini 2DE ImmunoblottingFrancisco-Jose Fernandez-Gomez 1, Fanny Jumeau 1,2, Maxime Derisbourg 1, Sylvie Burnouf 1, Hélène Tran 1, Sabiha Eddarkaoui 1, Hélène Obriot 1, Virginie Dutoit-Lefevre 3, Vincent Deramecourt 4, Valérie Mitchell 2, Didier Lefranc 3, Malika Hamdane 1, David Blum 1, Luc Buée 1, Valérie Buée-Scherrer 1, Nicolas Sergeant 1
1Team Alzheimer & Tauopathies, Jean-Pierre Aubert Research Centre, Inserm UMR 837, 2EA 4308-Department of Reproductive Biology-Spermiology-CECOS, CHRU-Lille, 3EA2686-Laboratorie d'Immunologie, Faculté de Médecine - Pôle Recherche, 4Department of Neurology, CHRU-Lille
A common protein extraction protocol using urea/thiourea/SDS buffer for human and mice brain tissue allows indentification of proteins by 2D-DIGE and their subsequent characterization by mini 2DE immunoblotting. This method enables one to obtain more reproducible and reliable results from human biopsies and experimental models.
