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Joint Bioenergy Institute

3 ARTICLES PUBLISHED IN JoVE

Biology

Histochemical Staining of Arabidopsis thaliana Secondary Cell Wall Elements

Prajakta Pradhan Mitra^1,2, Dominique Loqué^1,2

¹Feedstocks Division, Joint Bioenergy Institute, ²Physical Biosciences Division, Lawrence Berkeley National Laboratory

Plant cell wall composition varies between tissue types and can include lignin, cellulose, hemicelluloses, and pectin. Various staining techniques have been developed to visualize differences at the cell-type level. This paper is a compilation of commonly used cell wall staining techniques.

Biochemistry

Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE

Venkataramana R. Pidatala^*1,2, Amir Mahboubi^*1,2, Jenny C. Mortimer^1,2

¹Joint BioEnergy Institute, ²Environmental and Systems Biology, BioSciences Division, Lawrence Berkeley National Laboratory

A protocol for the structural analysis of polysaccharides by gel electrophoresis (PACE), using mannan as an example, is described.

Environment

Ecosystem Fabrication (EcoFAB) Protocols for The Construction of Laboratory Ecosystems Designed to Study Plant-microbe Interactions

Jian Gao^1,2, Joelle Sasse^1,2, Kyle M. Lewald^1,2, Kateryna Zhalnina^1,2, Lloyd T. Cornmesser^1,2, Todd A. Duncombe³, Yasuo Yoshikuni², John P. Vogel², Mary K. Firestone⁴, Trent R. Northen^1,2

¹Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, ²Joint Genome Institute, Department of Energy, ³Joint BioEnergy Institute, ⁴Department of Environmental Science Policy and Management, University of California

This article describes detailed protocols for ecosystem fabrication of devices (EcoFABs) that enable the studies of plants and plant-microbe interactions in highly controlled laboratory conditions.