Mapping the Cellular Distribution of an Optogenetic Protein Using a Light-Stimulation GridAlejandro Pizzoni 1, Nyla Naim 1,2, Xuefeng Zhang 1, Daniel L Altschuler 1
1Department of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, 2Addgene
This protocol involves transfecting cAMP sensors and bPAC-nLuc, an optogenetic protein, to accurately track its cellular distribution and response to light stimulation. The innovative approach of creating a cAMP response map using a point scanning system holds the potential for advancing research with optogenetic proteins in different fields.
