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Chongqing University

3 ARTICLES PUBLISHED IN JoVE

Neuroscience

Osmotic Pump-based Drug-delivery for In Vivo Remyelination Research on the Central Nervous System

Xiaorui Wang¹, Yixun Su^1,2, Xuelian Hu^1,3, Jianqin Niu¹

¹Department of Histology and Embryology, Chongqing Key Laboratory of Neurobiology, Brain, and Intelligence Research Key Laboratory of Chongqing Education Commission, Third Military Medical University, ²Research Centre, The Seventh Affiliated Hospital of Sun Yat-sen University, ³School of Medicine, Chongqing University

Demyelination takes place in multiple central nervous system diseases. A reliable in vivo drug delivery technique is necessary for remyelinating drug testing. This protocol describes an osmotic pump-based method that allows long-term drug delivery directly into the brain parenchyma and improves the drug bioavailability, with broad application in remyelination research.

Medicine

A Rat Orthotopic Renal Transplantation Model for Renal Allograft Rejection

Hang You^1,2, Xin Mao², Chenyang Wang², Gang Huang³, Marcus Groettrup^4,5, Jun Li²

¹School of Medicine, Chongqing University, ²Department of Urological Oncology Surgery, Chongqing University Cancer Hospital, ³Department of Biochemistry and Molecular Biology, College of Basic Medical Science, Army Medical University, ⁴Division of Immunology, Department of Biology, University of Konstanz, ⁵Biotechnology Institute Thurgau, University of Konstanz

The rat orthotopic renal transplantation model contributes to investigating the mechanism of renal allograft rejection. The current model increases the recipients' survival without interference with blood supply and venous reflux of the lower body using an end-to-end anastomosis of kidney implantation and an end-to-side "tunnel" method of ureter-bladder anastomosis.

Medicine

A Modified Cuff Technique for Mouse Cervical Heterotopic Heart Transplantation Model

Xin Mao^*1, Peng Xian^*1, Hang You^1,2, Gang Huang³, Jun Li¹

¹Department of Urological Oncology Surgery, Chongqing University Cancer Hospital, ²School of Medicine, Chongqing University, ³Department of Biochemistry and Molecular Biology, College of Basic Medical Science, Army Medical University

In the present protocol, a mouse heart transplantation model is used for investigating the mechanism of cardiac allograft rejection. In this heterotopic heart transplantation model, operation efficiency is improved, and the survival of cardiac grafts is ensured by a cervical end-to-end anastomosis of heart implantation using a modified Cuff technique.