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Agroscope

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)

¹Institute for Food Sciences IFS, Agroscope

Here, ribosomal spacer PCR (RS-PCR) is used together with a miniaturized electrophoresis system as a fast and high resolution method for genotyping S. aureus at moderate costs allowing a high throughput.

Environment

A Loop-mediated Isothermal Amplification (LAMP) Assay for Rapid Identification of Bemisia tabaci

Simon Blaser^1,2,3, Hanspeter Diem⁴, Andreas von Felten⁴, Morgan Gueuning¹, Michael Andreou⁵, Neil Boonham^6,7, Jennifer Tomlinson⁶, Pie Müller^2,3, Jürg Utzinger^2,3, Beatrice Frey¹, Jürg E. Frey¹, Andreas Bühlmann⁸

¹Department of Method Development and Analytics, Agroscope, ²Swiss Tropical and Public Health Institute, ³University of Basel, ⁴Swiss Federal Plant Protection Service, Federal Office for Agriculture, ⁵OptiGene Limited, ⁶Fera Science Limited, ⁷School of Natural and Environmental Sciences, Newcastle University, ⁸Department of Plants and Plant Products, Agroscope

This paper reports the protocol for a rapid identification assay for Bemisia tabaci based on loop-mediated isothermal amplification (LAMP) technology. The protocol requires minimal laboratory training and can, therefore, be implemented on-site at points of entry for plant imports such as seaports and airports.