Use of a Filter Cartridge for Filtration of Water Samples and Extraction of Environmental DNAMasaki Miya 1, Toshifumi Minamoto 2, Hiroki Yamanaka 3, Shin-ichiro Oka 4, Keiichi Sato 4, Satoshi Yamamoto 2, Tetsuya Sado 1, Hideyuki Doi 5
1Department of Ecology and Environmental Sciences, Natural History Museum and Institute, Chiba, 2Graduate School of Human Development and Environment, Kobe University, 3Faculty of Science and Technology, Ryukoku University, 4Okinawa Churashima Research Center, 5Graduate School of Simulation Studies, University of Hyogo
We describe a protocol for filtration of water samples with a filter cartridge and extraction of environmental DNA (eDNA) without having to cut open the housing to remove the filter. This protocol is developed for metabarcoding eDNA from fishes, but is also applicable to eDNA from other organisms.
