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University of Illinois at Springfield

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

In vitro Electroporation of the Lower Rhombic Lip of Midgestation Mouse Embryos

Patrick J. Holland¹, Angela M. George¹, Leslie T.C. Worrell¹, Rebecca L. Landsberg¹

¹Biology Department, University of Illinois at Springfield

This study describes the development of an in vitro electroporation technique that allows for the manipulation of gene expression in the lower rhombic lip of midgestation embryos.

Bioengineering

Surgical Retrieval, Isolation and In vitro Expansion of Human Anterior Cruciate Ligament-derived Cells for Tissue Engineering Applications

Ashim Gupta¹, Kevin Sharif², Megan Walters², Mia D. Woods¹, Anish Potty², Benjamin J. Main⁴, Saadiq F. El-Amin III^1,2,3

¹Department of Medical Microbiology, Immunology & Cell Biology, Southern Illinois University School of Medicine, ²Division of Orthopaedics and Rehabilitation, Department of Surgery, Southern Illinois University School of Medicine, ³Department of Electrical and Computer Engineering, Biomedical Engineering Program, Southern Illinois University Carbondale, ⁴University of Illinois at Springfield

For future applications as a patch to repair partial tears of the Anterior Cruciate Ligament (ACL), human ACL derived cells were isolated from tissue obtained during reconstructive procedures, expanded in vitro and grown on tissue engineered scaffolds. Cellular adhesion and morphology was then performed to confirm biocompatibility on scaffold surface.