UNAM

2 ARTICLES PUBLISHED IN JoVE

Biology

Polymerase Chain Reaction and Dot-Blot Hybridization for Leptospira Detection in Water Samples

Eduardo Delgadillo-Tellez¹, Beatriz Zavaleta-Villa¹, Daniel Atilano-López², Rigoberto Hernández-Castro³, Angélica Olivo-Díaz¹, Erika Margarita Carrillo-Casas¹

¹Department of Molecular Biology and Histocompatibility, General Hospital “Dr. Manuel Gea González”, ²Diagnostic Laboratory-Bacteriology, Leptospira Section, Department of Microbiology and Immunology, Veterinary Medicine Faculty, UNAM, ³Department of Ecology of Pathogens, General Hospital “Dr. Manuel Gea González”

In this study, a dot-blot application was designed to detect Leptospira from the three main clades in water samples. This method allows for the identification of minimal DNA quantities specifically targeted by a digoxigenin-labeled probe, easily detected by an anti-digoxigenin antibody. This approach is a valuable and satisfactory tool for screening purposes.

Immunology and Infection

Murine Model of Advanced Periodontitis Induced by Nylon Ligature in the Second Upper Molar

Betsaida J. Ortiz-Sánchez^1,2, Imelda Juárez Avelar^2,3, Miriam Rodriguez-Sosa²

¹Undergraduate Program of Dental Surgeon, Facultad de Estudios Superiores Iztacala (FES-Iztacala), Universidad Nacional Autónoma de Mexico (UNAM), ²Innate Immunity Laboratory, Biomedicine Research Unit (UBIMED), FES-Iztacala, UNAM, ³Doctoral Program in Biological Sciences, UNAM

This study describes a modified 6-0 nylon ligation method for inducing periodontitis in mice, which is highly reproducible and represents an alternative for researchers to study periodontal disease from its development to its pathological consequences.