In Situ Microscopy for Real-time Determination of Single-cell Morphology in BioprocessesAnna Maria Marbà-Ardébol 1, Joern Emmerich 2, Michael Muthig 2, Peter Neubauer 1, Stefan Junne 1
1Chair of Bioprocess Engineering, Department of Biotechnology, Technische Universität Berlin, 2SOPAT GmbH
A photo-optical in situ microscopy device was developed to monitor the size of single cells directly in the cell suspension. The real-time measurement is conducted by coupling the photo-optical sterilizable probe to an automated image analysis. Morphological changes appear with dependence on the growth state and cultivation conditions.
