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Osaka City University Graduate School of Medicine

3 ARTICLES PUBLISHED IN JoVE

Cancer Research

Generation of a Liver Orthotopic Human Uveal Melanoma Xenograft Platform in Immunodeficient Mice

Ken Kageyama^1,3, Shinji Ozaki^2,3, Takami Sato³

¹Department of Diagnostic and Interventional Radiology, Osaka City University Graduate School of Medicine, ²Department of Surgery, National Hospital Organization, Kure Medical Cancer Center, ³Department of Medical Oncology, Sidney Kimmel Cancer Center, Thomas Jefferson University

Orthotopic human liver metastatic uveal melanoma xenograft mouse models were created using surgical orthotopic implantation techniques with patient-derived tumor chunk and needle injection techniques with cultured human uveal melanoma cell lines.

Immunology and Infection

Lentiviral CRISPR/Cas9-Mediated Genome Editing for the Study of Hematopoietic Cells in Disease Models

Soichi Sano¹, Ying Wang¹, Megan A. Evans¹, Yoshimitsu Yura¹, Miho Sano¹, Hayato Ogawa¹, Keita Horitani¹, Heather Doviak¹, Kenneth Walsh¹

¹Hematovascular Biology Center, Robert M. Berne Cardiovascular Research Center, University of Virginia School of Medicine

Described are protocols for the highly efficient genome editing of murine hematopoietic stem and progenitor cells (HSPC) by the CRISPR/Cas9 system to rapidly develop mouse model systems with hematopoietic system-specific gene modifications.

JoVE Core

Bone Marrow Transplantation Procedures in Mice to Study Clonal Hematopoiesis

Eunbee Park¹, Megan A. Evans⁴, Heather Doviak⁴, Keita Horitani⁴, Hayato Ogawa⁴, Yoshimitsu Yura⁴, Ying Wang², Soichi Sano^3,4, Kenneth Walsh^1,4

¹Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, ²Department of Cardiology, Xinqiao Hospital, Army Medical University, ³Department of Cardiology, Osaka City University Graduate School of Medicine, ⁴Hematovascular Biology Center, Robert M. Berne Cardiovascular Research Center, University of Virginia School of Medicine

We describe three methods of bone marrow transplantation (BMT): BMT with total-body irradiation, BMT with shielded irradiation, and BMT method with no pre-conditioning (adoptive BMT) for the study of clonal hematopoiesis in mouse models.