Measurement of BK-polyomavirus Non-Coding Control Region Driven Transcriptional Activity Via Flow CytometryJohannes Korth 1,2, Helene Sertznig 2, Siegfried Moyrer 2, Adrian Atilla Nicolas Doevelaar 2,3, Timm Henning Westhoff 3, Nina Babel 3, Oliver Witzke 4, Andreas Kribben 1, Ulf Dittmer 2, Marek Widera 2
1Department of Nephrology, University of Duisburg-Essen, University Hospital Essen, 2Institute for Virology, University of Duisburg-Essen, University Hospital Essen, 3Center for Translational Medicine, Medical Department I, Marien Hospital Herne, University Hospital of the Ruhr-University of Bochum, 4Department of Infectious Diseases, University of Duisburg-Essen, University Hospital Essen Hufelandstr
In this manuscript, a protocol is presented to perform FACS-based measurement of BK-polyomavirus transcriptional activity by using HEK293T cells transfected with a bidirectional reporter plasmid expressing tdTomato and eGFP. This method further allows to quantitatively determine the influence of novel compounds on viral transcription.
