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Neuroscience Research Australia

3 ARTICLES PUBLISHED IN JoVE

Neuroscience

High-resolution In Vivo Manual Segmentation Protocol for Human Hippocampal Subfields Using 3T Magnetic Resonance Imaging

Julie Winterburn^1,2, Jens C. Pruessner³, Chavez Sofia^4,5, Mark M. Schira^6,7, Nancy J. Lobaugh^4,8, Aristotle N. Voineskos^5,9, M. Mallar Chakravarty^1,2

¹Institute of Biomaterials and Biomedical Engineering, University of Toronto, ²Computational Brain Anatomy Laboratory, Douglas Institute, McGill University, ³McGill Centre for Studies in Aging, McGill University, ⁴MRI Unit, Research Imaging Centre, Campbell Family Mental Health Research Institute, Centre for Addiction and Mental Health, ⁵Department of Psychiatry, University of Toronto, ⁶School of Psychology, University of Wollongong, ⁷Neuroscience Research Australia, ⁸Department of Medicine, University of Toronto, ⁹Kimel Family Translational Imaging Genetics Research Laboratory, Research Imaging Centre, Campbell Family Mental Health Research Institute, Centre for Addiction and Mental Health

The goal of this manuscript is to study the hippocampus and hippocampal subfields using MRI. The manuscript describes a protocol for segmenting the hippocampus and five hippocampal substructures: cornu ammonis (CA) 1, CA2/CA3, CA4/dentate gyrus, strata radiatum/lacunosum/moleculare, and subiculum.

Neuroscience

Imaging Serotonergic Fibers in the Mouse Spinal Cord Using the CLARITY/CUBIC Technique

Huazheng Liang^1,2, Emma Schofield¹, George Paxinos^1,2

¹Brain Structure and Function Group, Neuroscience Research Australia, ²School of Medical Sciences, The University of New South Wales

Supraspinal projections are important for pain perception and other behaviors, and serotonergic fibers are one of these fiber systems. The present study focused on the application of the combined CLARITY/CUBIC protocol to the mouse spinal cord in order to investigate the termination of these serotonergic fibers.

Biology

CUBIC Protocol Visualizes Protein Expression at Single Cell Resolution in Whole Mount Skin Preparations

Huazheng Liang^*1,2, Bassem Akladios^*1, Cesar P. Canales^*1, Richard Francis³, Edna H. Hardeman¹, Annemiek Beverdam¹

¹The School of Medical Sciences, University of New South Wales Australia, ²Neuroscience Research Australia, ³Biomedical Imaging Facility, University of New South Wales Australia

This report describes a CUBIC protocol to clarify full thickness mouse skin biopsies, and visualize protein expression patterns, proliferating cells, and sebocytes at the single cell resolution in 3D. This method enables accurate assessment of skin anatomy and pathology, and of abnormal epidermal phenotypes in genetically modified mouse lines.