New York University Grossman School of Medicine

High-resolution ultrasound can help streamline experiments requiring timed-pregnant mice by determining the state of pregnancy, gestational age, and pregnancy losses. Presented here is a protocol to illustrate methods to assess mouse pregnancies as well as potential pitfalls (image artifacts) that may mimic pregnancy.

Laboratory assays can leverage prognostic value from the longitudinal optical coherence tomography (OCT)-based multimodal imaging of age-related macular degeneration (AMD). Human donor eyes with and without AMD are imaged using OCT, color, near-infrared reflectance scanning laser ophthalmoscopy, and autofluorescence at two excitation wavelengths prior to tissue sectioning.

This protocol presents a detailed procedure to prepare four carbon-11 radiotracers by way of the “loop method” [¹¹C]methylation. We describe the procedure to synthesize [¹¹C]ER-176,  with data for three additional radiotracers: [¹¹C]MRB, [¹¹C]mHED, and [¹¹C]PiB. The loop method provides an efficient synthesis with increased radiochemical yields against traditional reaction vessel [¹¹C]methylation.