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The V. Akhundov National Scientific Research Medical Prophylactic Institute

2 ARTICLES PUBLISHED IN JoVE

Bioengineering

Preparation and Characterization of Graphene-Based 3D Biohybrid Hydrogel Bioink for Peripheral Neuroengineering

Asli Pinar Zorba Yildiz^1,2,4, Hakan Darici^3,4,5, Burcak Yavuz^2,4,6, Emrah Sefik Abamor², Ceren Ozdemir^2,5, Muge Elif Yasin⁴, Melahat Bagirova^2,7, Adil Allahverdiyev^2,7, Erdal Karaoz^3,4,5,8

¹Vocational School of Health Services, Istinye University, ²Chemical Metallurgy Faculty, Yildiz Technical University, ³Faculty of Medicine, Department of Histology & Embryology, Istinye University, ⁴3D Bioprinting Design & Prototyping R&D Center, Istinye University, ⁵Stem Cell, and Tissue Engineering R&D Center, Istinye University, ⁶Vocational School of Health Services, Altinbas University, ⁷The V. Akhundov National Scientific Research Medical Prophylactic Institute, ⁸Center for Stem Cells and Regenerative Medicine (LivMedCell), Liv Hospital

In this manuscript, we demonstrate the preparation of a biohybrid hydrogel bioink containing graphene for use in peripheral tissue engineering. Using this 3D biohybrid material, the neural differentiation protocol of stem cells is performed. This can be an important step in bringing similar biomaterials to the clinic.

Bioengineering

Formulating and Characterizing an Exosome-based Dopamine Carrier System

Burçak Yavuz^1,2, Hakan Darici^3,4,5,6, Aslı Pınar Zorba Yildiz^2,7, Emrah Şefik Abamor², Murat Topuzoğullari², Melahat Bağirova⁸, Adil Allahverdiyev⁸, Erdal Karaoz^3,4,5,6,9

¹Vocational School of Health Services/İstanbul, Altinbas University, ²Chemical Metallurgy Faculty, Department of Bioengineering, Yildiz Technical University, ³Faculty of Medicine, Department of Histology & Embryology, Istinye University, ⁴3D Bioprinting Design & Prototyping R&D Center, Istinye University, ⁵Stem Cell and Tissue Engineering R&D Center, Istinye University, ⁶Medicine Faculty, Istinye University, ⁷Vocational School of Health Services, Istinye University, ⁸The V. Akhundov National Scientific Research Medical Prophylactic Institute, ⁹Center for Stem Cells and Regenerative Medicine (LivMedCell), Liv Hospital

Here, we aimed to obtain a formulation by dopamine loading of the isolated exosomes of stem cells from Wharton's jelly mesenchymal stem cells. Exosome isolation and characterization, drug loading into the resulting exosomes, and the cytotoxic activity of the developed formulation are described in this protocol.