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Stem Cell Research Center, Childrens Hospital of Pittsburgh of UPMC

5 ARTICLES PUBLISHED IN JoVE

Biology

Isolating Stem Cells from Soft Musculoskeletal Tissues

Yong Li^1,2,3,4, Haiying Pan¹, Johnny Huard^1,2,3,4,5

¹Stem Cell Research Center, Childrens Hospital of Pittsburgh of UPMC, ²Department of Bioengineering, University of Pittsburgh, ³Department of Orthopedic Surgery, University of Pittsburgh, ⁴Department of Pathology, University of Pittsburgh, ⁵Department of Molecular Genetics & Biochemistry, University of Pittsburgh

Isolating adult stem cells from musculoskeletal soft tissues based on the cell's adherence speed to flask.

Biology

Isolation of Blood-vessel-derived Multipotent Precursors from Human Skeletal Muscle

William C.W. Chen¹, Arman Saparov^2,3, Mirko Corselli⁴, Mihaela Crisan⁵, Bo Zheng⁶, Bruno Péault^7,8, Johnny Huard⁹

¹Stem Cell Research Center, Department of Bioengineering and Orthopedic Surgery, University of Pittsburgh, ²Department of Orthopedic Surgery, University of Pittsburgh, ³Nazarbayev University Research and Innovation System, Nazarbayev University, ⁴Department of Orthopaedic Surgery, UCLA Orthopaedic Hospital and the Orthopaedic Hospital Research Center, University of California at Los Angeles, ⁵Department of Cell Biology, Erasmus MC Stem Cell Institute, ⁶OHSU Center for Regenerative Medicine, Oregon Health & Science University, ⁷Centre for Cardiovascular Science and MRC Centre for Regenerative Medicine, Queen's Medical Research Institute and University of Edinburgh, ⁸David Geffen School of Medicine and the Orthopaedic Hospital Research Center, University of California at Los Angeles, ⁹Stem Cell Research Center, Department of Orthopedic Surgery and McGowan Institute for Regenerative Medicine, University of Pittsburgh

Blood vessels within human skeletal muscle harbor several multi-lineage precursor populations that are ideal for regenerative applications. This isolation method allows simultaneous purification of three multipotent precursor cell populations respectively from three structural layers of blood vessels: myogenic endothelial cells from intima, pericytes from media, and adventitial cells from adventitia.

Developmental Biology

Isolation of Perivascular Multipotent Precursor Cell Populations from Human Cardiac Tissue

James E. Baily¹, William C.W. Chen^2,3, Nusrat Khan⁴, Iain R. Murray⁴, Zaniah N. González Galofre⁴, Johnny Huard^5,6, Bruno Péault^4,7

¹Centre for Cardiovascular Science, Queen's Medical Research Institute, University of Edinburgh, ²Department of Bioengineering and Orthopaedic Surgery, University of Pittsburgh, ³Research Laboratory of Electronics and Department of Biological Engineering, Massachusetts Institute of Technology, ⁴MRC Centre for Regenerative Medicine, University of Edinburgh, ⁵Stem Cell Research Center, Department of Orthopaedic Surgery, University of Pittsburgh, ⁶Department of Orthopaedic Surgery, University of Texas Health Science Center at Houston, ⁷Department of Orthopaedic Surgery, UCLA Orthopaedic Hospital, David Geffen School of Medicine, University of California at Los Angeles

Human cardiac tissue harbours multipotent perivascular precursor cell populations that may be suitable for myocardial regeneration. The technique described here allows for the simultaneous isolation and purification of two multipotent stromal cell populations associated with native blood vessels, i.e. CD146⁺CD34^- pericytes and CD34⁺CD146^- adventitial cells, from the human myocardium.

JoVE Core

The Generation of Closed Femoral Fractures in Mice: A Model to Study Bone Healing

Justin N. Williams¹, Yong Li¹, Anuradha Valiya Kambrath¹, Uma Sankar¹

¹Department of Anatomy and Cell Biology, Indiana University School of Medicine

The murine closed femoral fracture model is a powerful platform to study fracture healing and novel therapeutic strategies to accelerate bone regeneration. The goal of this surgical protocol is to generate unilateral closed femoral fractures in mice using an intramedullary steel rod to stabilize the femur.

Engineering

Measurement of the Hand Transmitted Vibration of the Human Hand Arm System During Operation of a Hand Tractor

Shiqing Lu¹, Rui Jiang², Xinyi Xiao³, Yong Li¹, Xia Huang¹, Kun Song¹, Changming Chen¹, Jun Ding¹

¹School of Mechanical Engineering, Chongqing University of Technology, ²College of Mechanical and Electrical Engineering, Wenzhou University, ³Mechanical and Manufacturing Engineering, Miami University

Here, we present a standardized method for measurement of the hand transmitted vibration from handles of a single-axle tractor with special reference to changes in grip force and vibration frequency.