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University Of California Santa Barbara

4 ARTICLES PUBLISHED IN JoVE

Bioengineering

Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules

Aaron A. Rowe¹, Ryan J. White¹, Andrew J. Bonham¹, Kevin W. Plaxco²

¹Department of Chemistry and Biochemistry, University Of California Santa Barbara, ²Department of Chemistry and Biochemistry, Program in BioMolecular Science and Engineering, University Of California Santa Barbara

"E-DNA" sensors, reagentless, electrochemical biosensors that perform well even when challenged directly in blood and other complex matrices, have been adapted to the detection of a wide range of nucleic acid, protein and small molecule analytes. Here we present a general procedure for the fabrication and use of such sensors.

Behavior

Large-Scale Gravitaxis Assay of Caenorhabditis Dauer Larvae

Caroline Ackley¹, Lindsey Washiashi¹, Ruchira Krishnamurthy¹, Joel H. Rothman¹

¹Molecular Cellular and Developmental Biology, University of California

The present protocol outlines methods for conducting a large-scale gravitaxis assay with Caenorhabditis dauer larvae. This protocol allows for better detection of gravitaxis behavior compared with a plate-based assay.

Endless Worms Most Beautiful: Current Methods For Using Nematodes To Study Evolutionary Developmental Biology

Chee Kiang Ewe¹, Pradeep M. Joshi², Joel H. Rothman²

¹Department of Neurobiology, Tel Aviv University, ²Department of Molecular, Cellular, Developmental Biology, University of California Santa Barbara

Endless Worms Most Beautiful: Current Methods For Using Nematodes To Study Evolutionary Developmental Biology

Bioengineering

Optical Photothermal Infrared-Fluorescence In Situ Hybridization (OPTIR-FISH)

Zhongyue Guo^*1, Yeran Bai^*2,3, Fátima C. Pereira⁴, Ji-Xin Cheng^1,5

¹Department of Biomedical Engineering, Boston University, ²Neuroscience Research Institute, Department of Molecular, Cellular, and Developmental Biology, University of California Santa Barbara, ³Photothermal Spectroscopy Corp., ⁴School of Biological Sciences, University of Southampton, ⁵Department of Electrical & Computer Engineering, Photonics Center, Boston University

Here, we present a protocol using optical photothermal infrared-fluorescence in situ hybridization (OPTIR-FISH), also known as mid-infrared photothermal-FISH (MIP-FISH), to identify individual cells and understand their metabolism. This methodology can be applied broadly for diverse applications, including mapping cellular metabolism with single-cell resolution.