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University of Hawaii

2 ARTICLES PUBLISHED IN JoVE

Bioengineering

Introduction to the Ultrasound Targeted Microbubble Destruction Technique

Chad B. Walton¹, Cynthia D. Anderson¹, Rachel Boulay¹, Ralph V. Shohet¹

¹Department of Medicine, JABSOM, University of Hawaii

Ultrasound Targeted Microbubble Destruction (UTMD) can be used to direct site-specific delivery of bioactive molecules, including therapeutic genes, to target organs accessible to ultrasound, such as the heart and liver^1-6.

Biology

A Semi-Automated Workflow for the Cryopreservation of Coral Sperm to Support Biobanking and Aquaculture

Jonathan Daly^1,2, Rebecca Hobbs¹, Nikolas Zuchowicz³, Mary Hagedorn^4,5, Justine K. O’Brien^1,2

¹Taronga Institute of Science and Learning, Taronga Conservation Society Australia, ²School of Biological, Earth and Environmental Sciences, University of New South Wales, ³Department of Mechanical Engineering, University of Minnesota, ⁴Hawaii Institute of Marine Biology, University of Hawaii, ⁵Smithsonian National Zoo and Conservation Biology Institute

This protocol describes a semi-automated pathway to improve the efficiency and capacity of processing and cryopreservation of sperm from threatened coral species, aiming to secure genetic diversity and support reef restoration efforts.