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University of Minnesota Medical School

4 ARTICLES PUBLISHED IN JoVE

Biology

Pharmacological and Functional Genetic Assays to Manipulate Regeneration of the Planarian Dugesia japonica

¹Department of Pharmacology and The Stem Cell Institute, University of Minnesota Medical School

An attractive model for studying stem cell differentiation within a live animal is the planarian flatworm. Regeneration is studied by simple amputation experiments that are easily performed in a basic laboratory and are amenable to pharmacological and genetic (in vivo RNAi) manipulation as detailed by protocols in this article.

JoVE Journal

Isolation, Culture, and Transplantation of Muscle Satellite Cells

Norio Motohashi¹, Yoko Asakura¹, Atsushi Asakura¹

¹Stem Cell Institute, Paul and Sheila Wellstone Muscular Dystrophy Center, Department of Neurology, University of Minnesota Medical School

The isolation and culture of a pure population of quiescent satellite cells, a muscle stem cell population, is essential to the understanding of muscle stem cell biology and regeneration, as well as stem cell transplantation for therapies in muscular dystrophy and other degenerative diseases.

Biology

An In Vitro Model for Studying Cellular Transformation by Kaposi Sarcoma Herpesvirus

Shane C. McAllister¹, Ryan S. Hanson¹, Kyleen N. Grissom¹, Sara Botto², Ashlee V. Moses²

¹Division of Pediatric Infectious Diseases, University of Minnesota Medical School, ²Vaccine and Gene Therapy Institute, Oregon Health and Science University

Kaposi sarcoma (KS) is a tumor induced by infection with the oncogenic virus human herpesvirus-8/KS herpesvirus (HHV-8/KSHV). The endothelial cell culture model described here is uniquely suited for studying the mechanisms by which KSHV transforms host cells.

Medicine

Technical Considerations and Approach to Redo Foregut Surgery

David J. Leishman¹, Divinemercy Bakare², Madhuri Rao¹

¹Department of Surgery, Division of Thoracic and Foregut Surgery, University of Minnesota, ²University of Minnesota Medical School

Redo foregut surgery is associated with increased patient morbidity and presents a technical challenge for the surgeon. We describe our approach and considerations when performing a redo hiatal hernia repair to provide a guide for other surgeons and improve patient outcomes.