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Mayo Clinic

3 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Antimicrobial Susceptibility Testing of Mycobacterium Tuberculosis Complex for First and Second Line Drugs by Broth Dilution in a Microtiter Plate Format

Leslie Hall¹, Kurt P. Jude¹, Shirley L. Clark¹, Nancy L. Wengenack¹

¹Department of Laboratory Medicine and Pathology, Mayo Clinic

Antimicrobial susceptibility testing of Mycobacterium tuberculosis is challenging but critical for patient care. This microtiter plate format offers testing of 12 antimycobacterial drugs and provides minimum inhibitory concentration (MIC) values, which will aid in appropriate treatment.

Medicine

Assessment of Gastric Emptying in Non-obese Diabetic Mice Using a [¹³C]-octanoic Acid Breath Test

Christopher T. Creedon¹, Pieter-Jan Verhulst¹, Kyoung M. Choi¹, Jessica E. Mason¹, David R. Linden¹, Joseph H. Szurszewski¹, Simon J. Gibbons¹, Gianrico Farrugia¹

¹Enteric Neuroscience Program, Department of Physiology and Biomedical Engineering, Mayo Clinic

Determination of gastric emptying with a non-invasive [¹³C]-octanoic acid breath test for tracking gastroparesis in female NOD LtJ mice.

Biology

Whole Cell Electrophysiology of Primary Cultured Murine Enterochromaffin Cells

Katilyn Knutson^*1, Peter R. Strege^*1, Joyce Li², Andrew B. Leiter², Gianrico Farrugia¹, Arthur Beyder¹

¹Enteric Neuroscience Program, Division of Gastroenterology & Hepatology,Department of Physiology & Biomedical Engineering, Mayo Clinic, ²Division of Gastroenterology, Department of Medicine, University of Massachusetts Medical School

Enterochromaffin (EC) cells comprise a small subset of gastrointestinal epithelial cells. EC cells are electrically excitable and release serotonin, yet difficulties in culturing and identifying EC cells have limited physiological studies. The method presented here establishes a primary culture model amenable to examination of single EC cells by electrophysiology.