Production and Purification of Non Replicative Canine Adenovirus Type 2 Derived VectorsMarion Szelechowski 1, Corinne Bergeron 2, Daniel Gonzalez-Dunia 1, Bernard Klonjkowski 2
1INSERM UMR 1043, CNRS UMR 5282, Université Toulouse 3, 2UMR Viroligie, INRA ENVA ANSES
These past 15 years, canine adenovirus type 2 (CAV2)-derived vectors have proven their efficiency to transduce cells in vitro and in vivo and are widely used for vaccination and gene therapy. Here, we describe a procedure to construct, produce and purify CAV2 vectors, giving rise to high-titer viral suspensions.
