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Royal Institute of Technology (KTH)

2 ARTICLES PUBLISHED IN JoVE

Environment

Transcript and Metabolite Profiling for the Evaluation of Tobacco Tree and Poplar as Feedstock for the Bio-based Industry

Colin Ruprecht¹, Takayuki Tohge¹, Alisdair Fernie¹, Cara L. Mortimer², Amanda Kozlo², Paul D. Fraser², Norma Funke¹, Igor Cesarino^3,4, Ruben Vanholme^3,4, Wout Boerjan^3,4, Kris Morreel^3,4, Ingo Burgert^5,6, Notburga Gierlinger^5,6, Vincent Bulone⁷, Vera Schneider⁸, Andrea Stockero⁸, Juan Navarro-Aviñó⁹, Frank Pudel¹⁰, Bart Tambuyser¹¹, James Hygate¹², Jon Bumstead¹³, Louis Notley¹³, Staffan Persson^1,14

¹Max Planck Institute for Molecular Plant Physiology, ²School of Biological Sciences, Plant Molecular Science, Centre for Systems and Synthetic Biology, Royal Holloway, University of London, ³Department of Plant Systems Biology, VIB, ⁴Department of Plant Biotechnology and Bioinformatics, UGhent, ⁵Institute for Building Materials, ETH Zurich, ⁶Applied Wood Materials, EMPA, ⁷Division of Glycoscience, School of Biotechnology, AlbaNova University Center, Royal Institute of Technology (KTH), ⁸European Research and Project Office GmbH, ⁹ABBA Gaia S.L., ¹⁰Pflanzenöltechnologie, ¹¹Capax Environmental Services, ¹²Green Fuels, ¹³Neutral Consulting Ltd, ¹⁴Plant Cell Biology Research Centre, School of Botany, University of Melbourne

Plant biomass offers a renewable resource for multiple products, including fuel, feed, food, and a variety of materials. In this paper we investigate the properties of tobacco tree (Nicotiana glauca) and poplar as suitable sources for a biorefinery pipeline.

Biology

Scanning Electron Microscopy (SEM) Protocols for Problematic Plant, Oomycete, and Fungal Samples

M. Angélica Bello¹, Yolanda Ruiz-León², J. Vladimir Sandoval-Sierra³, Svetlana Rezinciuc⁴, Javier Diéguez-Uribeondo³

¹Biodiversity and Conservation Department, Real Jardín Botánico, CSIC, ²Research Support Unit, Real Jardín Botánico, CSIC, ³Mycology Department, Real Jardín Botánico, CSIC, ⁴Division of Glycoscience, AlbaNova University Center, Royal Institute of Technology (KTH)

Problems in the processing of biological samples for scanning electron microscopy observation include cell collapse, treatment of samples from wet microenvironments and cell destruction. Low-cost and relatively rapid protocols suited for preparing challenging samples such as floral meristems, oomycete cysts, and fungi (Agaricales) are compiled and detailed here.