Ex Vivo Imaging of Postnatal Cerebellar Granule Cell Migration Using Confocal MacroscopyMagalie Bénard 1, Alexis Lebon 1, Hitoshi Komuro 2, David Vaudry 1, Ludovic Galas 1
1PRIMACEN, Cell Imaging Platform of Normandy, Inserm, IRIB, University of Rouen, 2Department of Neurobiology, School of Medicine, Yale University
During postnatal cerebellum development, immature granule cells originating from the germinal zone exhibit distinct modalities of migration to reach their final destination and to establish neuronal networks. This protocol describes the preparation of cerebellar slices and the confocal macroscopic approach used to investigate the factors that regulate neuronal migration.
