Employing Digital Droplet PCR to Detect BRAF V600E Mutations in Formalin-fixed Paraffin-embedded Reference Standard Cell LinesNirmal Rajasekaran 1, Myung Ryurl Oh 3, Sung-Su Kim 1, Si Eun Kim 3, Young Deug Kim 4, Hyun-Jeung Choi 2, Bohyun Byun 2, Young Kee Shin 1,2
1Research Institute of Pharmaceutical Science, Department of Pharmacy, College of Pharmacy, Seoul National University, 2The Center for Anti-Cancer CDx, N-Bio, Seoul National University, 3ABION CRO, 4ABION Inc., R&D Center
The goal of this video is to demonstrate how to perform automated DNA extraction from formalin-fixed paraffin-embedded (FFPE) reference standard cell lines and digital droplet PCR (ddPCR) analysis to detect rare mutations in a clinical setting. Detecting mutations in FFPE samples demonstrates the clinical utility of ddPCR in FFPE samples.
