Generation and Culture of Blood Outgrowth Endothelial Cells from Human Peripheral BloodMark L. Ormiston 1, Mark R. Toshner 2, Fedir N. Kiskin 1, Christopher J. Z. Huang 1, Emily Groves 1, Nicholas W. Morrell 1, Amer A. Rana 1
1Department of Medicine, University of Cambridge, 2Papworth Hospital
This protocol allows for the reliable generation and characterization of blood outgrowth endothelial cells (BOECs) from a small volume of adult peripheral blood. BOECs can be used as a surrogate for endothelial cells from patients with vascular disorders and as a substrate for the generation of induced pluripotent stem cells.
