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Migal-Galilee Research Institute

1 ARTICLES PUBLISHED IN JoVE

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Water in Oil Emulsions: A New System for Assembling Water-soluble Chlorophyll-binding Proteins with Hydrophobic Pigments
Dominika Bednarczyk 1, Dror Noy 2
1Department of Biological Chemistry, Weizmann Institute of Science, 2Migal-Galilee Research Institute

This manuscript describes a simple and high-throughput method for assembling water-soluble proteins with hydrophobic pigments that is based on water-in-oil emulsions. We demonstrate the effectiveness of the method in the assembly of native chlorophylls with four variants of recombinant water-soluble-chlorophyll binding proteins (WSCPs) of Brassica plants expressed in E. coli.

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