Using X-ray Crystallography, Biophysics, and Functional Assays to Determine the Mechanisms Governing T-cell Receptor Recognition of Cancer AntigensBruce J. MacLachlan *1, Alexander Greenshields-Watson *1, Georgina H Mason *1, Andrea J Schauenburg 1, Valentina Bianchi 1,2,3, Pierre J Rizkallah 1, Andrew K Sewell 1, Anna Fuller 1, David K Cole 1
1Division of Infection and Immunity and Systems Immunity Research Institute, Cardiff University, 2Department of Oncology, University Hospital of Lausanne (CHUV), 3Ludwig Insitutue for Cancer Research, Lausanne Branch, University of Lausanne
Here, we describe methods that we commonly employ in the laboratory to determine how the nature of the interaction between the T-cell receptor and tumor antigens, presented by human leukocyte antigens, governs T-cell functionality; these methods include protein production, X-ray crystallography, biophysics, and functional T-cell experiments.
