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Hôpital Européen Georges Pompidou

2 ARTICLES PUBLISHED IN JoVE

JoVE Core

Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR

Riccardo Balzan¹, Laetitia Fernandes¹, Arnaud Comment², Laetitia Pidial³, Bertrand Tavitian³, Paul R. Vasos¹

¹LCBPT - UMR8601, Institut de Chimie, Université Paris Descartes, ²Institute of Physics of Biological Systems, EPFL, ³PARCC - INSERM U970, Université Paris Descartes

The sensitivity enhancement provided by dissolution dynamic nuclear polarization (DNP) enables following metabolic processes in real time by NMR and MRI. The characteristics and performances of a dedicated dissolution DNP setup designed for study enzymatic reactions are discussed.

Medicine

ADSC-sheet Transplantation to Prevent Stricture after Extended Esophageal Endoscopic Submucosal Dissection

Guillaume Perrod^1,2,3, Laetitia Pidial³, Sophie Camilleri^1,4, Alexandre Bellucci^1,3,5, Amaury Casanova³, Thomas Viel³, Bertrand Tavitian^1,3,5, Chirstophe Cellier^1,2, Olivier Clément^1,3,5, Gabriel Rahmi^1,2,3

¹Assistance Publique-Hôpitaux de Paris, Université Paris Descartes Sorbonne Paris Cité, ²Department of Gastroenterology, Hôpital Européen Georges Pompidou, ³UMR-S970, Université Paris Descartes Sorbonne Paris Cité, ⁴Department of Pathology, Hôpital Européen Georges Pompidou, ⁵Department of Radiology, Hôpital Européen Georges Pompidou

This study reports a successful method of endoscopical adipose tissue-derived stromal cell (ADSC)-sheet transplantation for esophageal stricture prevention after an extended endoscopic submucosal dissection (ESD) in a swine model.