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Center for Neurodegenerative Science Van Andel Institute

1 ARTICLES PUBLISHED IN JoVE

Biochemistry

Multimer-PAGE: A Method for Capturing and Resolving Protein Complexes in Biological Samples

Tyler Rhinesmith¹, Bryan A. Killinger², Akhil Sharma³, Anna Moszczynska³

¹Physiology, Michigan State University, ²Center for Neurodegenerative Science Van Andel Institute, ³Pharmaceutical Sciences, Wayne State University

A method for stabilizing and separating native protein complexes from unmodified tissue lysate using an amine-reactive protein cross-linker coupled to a novel two-dimensional polyacrylamide gel electrophoresis (PAGE) system is presented.