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Istituto Superiore di Sanità

3 ARTICLES PUBLISHED IN JoVE

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Immunology and Infection

Magnetic Stirrer Method for the Detection of Trichinella Larvae in Muscle Samples
Anne Mayer-Scholl 1, Edoardo Pozio 2, Jennifer Gayda 1, Nora Thaben 1, Peter Bahn 1, Karsten Nöckler 1
1Federal Institute for Risk Assessment, 2Istituto Superiore di Sanità

The prevention of human trichinellosis in many countries worldwide is based on the laboratory examination of muscle samples from susceptible animals by methods of digestion, of which the magnetic stirrer method is considered the gold standard.

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Immunology and Infection

Microfluidic Co-Culture Models for Dissecting the Immune Response in in vitro Tumor Microenvironments
Adele De Ninno 1, Francesca Romana Bertani 1, Annamaria Gerardino 1, Giovanna Schiavoni 2, Martina Musella 3, Claudia Galassi 3, Fabrizio Mattei 2, Antonella Sistigu 3,4, Luca Businaro 1
1CNR Institute for Photonics and Nanotechnology, 2Dept. of Oncology and Molecular Medicine, Istituto Superiore di Sanità, 3Istituto di Patologia Generale, Università Cattolica del Sacro Cuore, 4Tumor Immunology and Immunotherapy Unit, IRCCS Regina Elena National Cancer Institute

In the age of immunotherapy and single-cell genomic profiling, cancer biology requires novel in vitro and computational tools for investigating the tumor-immune interface in a proper spatiotemporal context. We describe protocols to exploit tumor-immune microfluidic co-cultures in 2D and 3D settings, compatible with dynamic, multiparametric monitoring of cellular functions.

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Biology

High-Quality Brain and Bone Marrow Nuclei Preparation for Single Nuclei Multiome Assays
Carolina Moraes Cabé 1, Sophie Novault 1, Ana Jeemin Choi 2, Valerie Seffer 1, Laura Barrio Cano 1, Valentina Libri 1,3, Milena Hasan 1
1Cytometry and Biomarkers UTechS, C2RT, Institut Pasteur, Université Paris Cité, 2Microenvironment and Immunity Unit, Institut Pasteur, Université Paris Cité, INSERM U1224, 3Istituto Superiore di Sanità

The success of single-cell/single-nuclei transcriptomics and multi-omics largely depends on the quality of cells/nuclei. Therefore, isolating cells/nuclei from tissue and their purification must be highly standardized. This protocol describes the preparation of nuclei from the brain and bone marrow for downstream single-nuclei multiome assay.

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