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Maastricht UMC+

1 ARTICLES PUBLISHED IN JoVE

Bioengineering

Visualizing Angiogenesis by Multiphoton Microscopy In Vivo in Genetically Modified 3D-PLGA/nHAp Scaffold for Calvarial Critical Bone Defect Repair

Jian Li¹, Holger Jahr^2,3, Wei Zheng⁴, Pei-Gen Ren¹

¹Center for Translational Medicine Research and Development, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, ²Department of Orthopedic Surgery, Maastricht UMC+, ³Department of Orthopaedic Surgery, University Hospital RWTH, ⁴Research Laboratory for Biomedical Optics and Molecular Imaging, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences

Here, we present a protocol to visualize blood vessel formation in vivo and in real-time in 3D scaffolds by multiphoton microscopy. Angiogenesis in genetically modified scaffolds was studied in a murine calvarial critical bone defect model. More new blood vessels were detected in the treatment group than in controls.