Simultaneous Measurement of HDAC1 and HDAC6 Activity in HeLa Cells Using UHPLC-MSClaudia A. Simões-Pires *1, Vincent Zwick *1, Sylvian Cretton 1, Muriel Cuendet 1
1Section des Sciences Pharmaceutiques, Universités des Geneva–Lausanne (EPGL)
The present method serves to identify isoform-specific inhibitors of histone deacetylases (HDAC) in HeLa cells by the UHPLC-MS analysis of multiple substrates. This is an antibody-free method developed to reflect HDAC1 and HDAC6 activity in the living cell environment, in contrast to single-isoform cell-free assays.
