Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay MethodGail M Nelson 1, Jenna M Guynn 2, Brian N Chorley 1
1National Health and Environmental Effects Research Laboratory, U.S. Environmental Protection Agency, 2Oak Ridge Institute for Science and Education at U.S. Environmental Protection Agency
A capillary-based immunoassay using a commercial platform to measure target proteins from total protein preparations is demonstrated. In addition, assay parameters of exposure time, protein concentration, and antibody dilution are optimized for a cell culture model system.
