Processing of Human Cardiac Tissue Toward Extracellular Matrix Self-assembling Hydrogel for In Vitro and In Vivo ApplicationsMatthias Becker 1,2, Janita A. Maring 1,2, Barbara Oberwallner 2, Benjamin Kappler 2, Oliver Klein 1,2, Volkmar Falk 2,3,4,5, Christof Stamm 2,3,4
1Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 2Berlin-Brandenburg Center for Regenerative Therapies (BCRT), 3German Center for Cardiovascular Research (DZHK), 4Deutsches Herzzentrum Berlin (DHZB), 5Department of Cardiovascular Surgery, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health
This protocol describes the complete decellularization of human myocardium while preserving its extracellular matrix components. Further processing of the extracellular matrix results in the production of microparticles and a cytoprotective self-assembling hydrogel.
