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École Polytechnique Fédérale de Lausanne (EPFL)

6 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Single-Cell Quantification of Protein Degradation Rates by Time-Lapse Fluorescence Microscopy in Adherent Cell Culture

Andrea Brigitta Alber¹, David Michael Suter¹

¹UPSUTER, Institute of Bioengineering (IBI), School of Life Sciences, École Polytechnique Fédérale de Lausanne (EPFL)

This protocol describes a method to determine protein half-lives in single living adherent cells, using pulse labeling and fluorescence time-lapse imaging of SNAP-tag fusion proteins.

Chemistry

Electrophoretic Crystallization of Ultrathin High-performance Metal-organic Framework Membranes

Guangwei He¹, Deepu J. Babu¹, Kumar Varoon Agrawal¹

¹Laboratory of Advanced Separations (LAS), École Polytechnique Fédérale de Lausanne (EPFL)

A simple, reproducible, and versatile approach for the synthesis of intergrown, polycrystalline metal-organic framework membranes on a wide range of unmodified porous and non-porous supports is presented.

Chemistry

A Continuous-flow Photocatalytic Reactor for the Precisely Controlled Deposition of Metallic Nanoparticles

Sina Abdolhosseinzadeh^1,2, Mirsajjad Mousavi³, Navid Haghmoradi³, Selmiye Alkan Gürsel^3,4

¹Institut des matériaux, École Polytechnique Fédérale de Lausanne (EPFL), ²Laboratory for Functional Polymers, Swiss Federal Laboratories for Materials Science and Technology (Empa), ³Faculty of Engineering and Natural Sciences, Sabanci University, ⁴SUNUM Nanotechnology Research Centre, Sabanci University

For a continuous and scalable synthesis of noble-metal-based nanocomposites, a novel photocatalytic reactor is developed and its structure, operation principles, and product quality optimization strategies are described.

Neuroscience

Dynamic Inter-subject Functional Connectivity Reveals Moment-to-Moment Brain Network Configurations Driven by Continuous or Communication Paradigms

Thomas A.W. Bolton^1,2, Delphine Jochaut³, Anne-Lise Giraud³, Dimitri Van De Ville^1,2

¹Institute of Bioengineering, École Polytechnique Fédérale de Lausanne (EPFL), ²Department of Radiology and Medical Informatics, University of Geneva, ³Department of Neuroscience, University of Geneva

The goal of the described approach is to determine at what moments of the paradigm (temporal perspective), and between which regions (spatial perspective), significant reconfigurations in functional connectivity occur on functional magnetic resonance imaging recordings during which a time-locked stimulus is played.

Environment

Flash Infrared Annealing for Perovskite Solar Cell Processing

Pui Sha Victoria Ling^1,2, Anders Hagfeldt², Sandy Sanchez²

¹Department of Chemistry, Molecular Sciences Research Hub, Imperial College London, ²Laboratory of Photomolecular Science (LSPM), École Polytechnique Fédérale de Lausanne (EPFL)

We describe a flash infrared annealing method used for the synthesis of perovskite and mesoscopic-TiO₂ films. Annealing parameters are varied and optimized for processing on fluorine-doped tin oxide (FTO) glass and indium tin oxide-coated polyethylene terephthalate (ITO PET), subsequently giving devices power conversion efficiencies >20%.

Developmental Biology

Preparation of Single-Somite Explants from Zebrafish Embryos

Sundar R. Naganathan¹, Andrew C. Oates¹

¹Institute of Bioengineering, École polytechnique fédérale de Lausanne (EPFL)

We present a protocol for isolating single somites from zebrafish embryos, the dynamics of which can be followed in culture for several hours by fluorescence time-lapse microscopy, thus providing a methodology to quantify tissue-scale shape changes at single-cell resolution.