Assessment of the Cytotoxic and Immunomodulatory Effects of Substances in Human Precision-cut Lung SlicesVanessa Neuhaus *1, Olga Danov *1, Sebastian Konzok 1, Helena Obernolte 1, Susann Dehmel 1, Peter Braubach 2, Danny Jonigk 2, Hans-Gerd Fieguth 3, Patrick Zardo 4, Gregor Warnecke 4, Christian Martin 5, Armin Braun 1,6, Katherina Sewald 1
1Fraunhofer Institute for Toxicology and Experimental Medicine (ITEM), German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Member of REBIRTH Cluster of Excellence, 2Institute for Pathology, Hannover Medical School, German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), 3Division of Thoracic and Vascular Surgery, Klinikum Region Hannover (KRH), 4Department of Cardiothoracic, Transplantation and Vascular Surgery (HTTG), Hannover Medical School, German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), 5Institute of Pharmacology and Toxicology, RWTH Aachen University, 6Institute for Immunology, Hannover Medical School
In view of the 3Rs principle, respiratory models as alternatives to animal studies are evolving. Especially for risk assessment of respiratory substances, there is a lack of appropriate assays. Here, we describe the use of human precision-cut lung slices for the assessment of airborne substances.
