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Leprino Foods, Inc

2 ARTICLES PUBLISHED IN JoVE

Environment

Colorimetric Paper-based Detection of Escherichia coli, Salmonella spp., and Listeria monocytogenes from Large Volumes of Agricultural Water

Bledar Bisha¹, Jaclyn A. Adkins², Jana C. Jokerst³, Jeffrey C. Chandler¹, Alma Pérez-Méndez⁴, Shannon M. Coleman⁴, Adrian O. Sbodio⁵, Trevor V. Suslow⁵, Michelle D. Danyluk⁶, Charles S. Henry², Lawrence D. Goodridge⁷

¹Department of Animal Science, University of Wyoming, ²Department of Chemistry, Colorado State University, ³Department of Environmental and Radiological Health Sciences, Colorado State University, ⁴Department of Animal Sciences, Colorado State University, ⁵Department of Plant Sciences, University of California, Davis, ⁶Department of Food Science and Human Nutrition, Citrus Research and Education Center, University of Florida, ⁷Department of Food Science and Agricultural Chemistry, McGill University

A protocol involving integrated concentration, enrichment, and end-point colorimetric detection of foodborne pathogens in large volumes of agricultural water is presented here. Water is filtered through Modified Moore Swabs (MMS), enriched with selective or non-selective media, and detection is performed using paper-based analytical devices (µPAD) imbedded with bacterial-indicative colorimetric substrates.

Environment

Detection of Viruses from Bioaerosols Using Anion Exchange Resin

Joshua W. Schaeffer¹, Jeffrey C. Chandler², Margaret Davidson^1,3, Sheryl L. Magzamen¹, Alma Pérez-Méndez⁴, Stephen J. Reynolds¹, Lawrence D. Goodridge⁵, John Volckens⁶, Alan B. Franklin², Susan A. Shriner², Bledar Bisha⁷

¹High Plains Intermountain Center for Agricultural Health and Safety, Department of Environmental and Radiological Health Sciences, Colorado State University, ²National Wildlife Research Center, Wildlife Services, Animal and Plant Health Inspection Service, United States Department of Agriculture, ³Western Sydney University, ⁴Leprino Foods, Inc, ⁵Department of Food Science and Agricultural Chemistry, McGill University, ⁶Department of Mechanical Engineering, Colorado State University, ⁷Department of Animal Science, University of Wyoming

An anion exchange resin-based method, adapted to liquid impingement-based bioaerosol sampling of viruses is demonstrated. When coupled with downstream molecular detection, the method allows for facile and sensitive detection of viruses from bioaerosols.