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University of Milan-Bicocca Medical School

2 ARTICLES PUBLISHED IN JoVE

Medicine

Endotoxin Activity Assay for the Detection of Whole Blood Endotoxemia in Critically Ill Patients

Riccardo Pinciroli^1,2, Simone Checchi², Maurizio Bottiroli¹, Gianpaola Monti¹, Giampaolo Casella¹, Roberto Fumagalli^1,2

¹Department of Anesthesia and Critical Care, Niguarda Hospital, ²University of Milan-Bicocca Medical School

We hereby present a protocol to measure at the bedside the endotoxin activity of human whole blood samples. The Endotoxin Activity assay is a simple test to perform and may be a useful biomarker in critically ill patients with sepsis.

Medicine

A Novel Inhalation Mask System to Deliver High Concentrations of Nitric Oxide Gas in Spontaneously Breathing Subjects

Riccardo Pinciroli¹, Lisa Traeger¹, Anna Fischbach¹, Stefano Gianni¹, Caio Cesar Araujo Morais¹, Bijan Safaee Fakhr¹, Raffaele Di Fenza¹, Dianna Robinson³, Ryan Carroll², Warren M. Zapol¹, Lorenzo Berra¹

¹Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital and Harvard Medical School, ²Division of Pediatric Critical Care Medicine, Department of Pediatrics, Massachusetts General Hospital and Harvard Medical School, ³Respiratory Care, Massachusetts General Hospital

This simple and highly adaptable system device for the inhalation of high-concentration nitric oxide (NO) gas does not require mechanical ventilators, positive pressure, or high gas flows. Standard medical consumables and a snug-fitting mask are used to safely deliver NO gas to spontaneously breathing subjects.